Pozivamo Vas na predavanje

U petak, 11. lipnja 2010. u 9:30 sati na Institutu Ruđer Bošković, Bijenička 54, Zagreb u Seminaru Zavoda za molekularnu biologiju V krilo, soba 201 održat će se predavanje:

Dr. Karin Krupinska
Institute of Botany, Christian-Albrechts-Universität zu Kiel, Germany

IN CHLOROPLASTS THE PLASTID-NUCLEUS TARGETED DNA BINDING PROTEIN Whirly1 FUNCTIONS AS A REGULATOR OF PHOTOSYSTEM I

Whirly1 is a single stranded DNA binding protein targeted to both plastids (Krause et al. 2005) and the nucleus (Grabowski et al. 2008). Immunogold labeling showed that plastid localized Whirly1 is partly associated to stroma exposed thylakoid membranes. In order to learn more about the function of plastid located Whirly1, the interaction partners of Whirly1 were identified by screening of a cDNA expression library of Arabidopsis leaves via the yeast two hybrid system. Among the ten putative interaction partner proteins identified one is the Lhca1 protein, which belongs to the light harvesting complex of photosystem I (LHCI). Blue native gel electrophoresis revealed that photosystem I conformation is changed in T-DNA insertion mutants of Arabidopis thaliana and in Whirly1 knockdown plants of barley. Preliminary results on functional analysis of photosystem I in the mutants suggest that Whirly1 plays a role in the regulation of cyclic electron transport.

References:
Grabowski E, Miao Y, Mulisch M, Krupinska K (2008) Whirly1 in barley leaves is located in chloroplasts and nuclei of the same cell. Plant Physiology 147: 1-5
Krause K, Kilbienski I, Mulisch M, Rödiger A, Schäfer A, Krupinska K (2005) DNA-binding proteins of the Whirly family in Arabidopsis thaliana are targeted to the organelles. FEBS Lett 579: 3707-3712

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